Branched DNA assay
Branched DNA assay
The Branched DNA assay (pronounced: /ˈbræntʃt ˈdiː.enˈeɪ æˈseɪ/) is a molecular biological technique used to measure DNA quantity in a sample. The term originates from the structure of the DNA used in the assay, which is branched rather than linear.
History
The Branched DNA assay was first developed in the 1980s by Chiron Corporation, a biotechnology firm. The technique was initially used for the detection of Hepatitis B virus in blood samples.
Procedure
The Branched DNA assay involves the use of a probe that binds to a specific sequence of the target DNA. This probe is attached to a branched DNA molecule, which is then detected and quantified using a luminescent reaction. The amount of light produced is proportional to the amount of target DNA in the sample.
Applications
The Branched DNA assay is primarily used in clinical diagnostics for the quantification of viral load in patients with viral infections such as HIV and Hepatitis C. It is also used in genetic research for the quantification of specific DNA sequences.
Related Terms
- Molecular biology
- DNA
- Probe
- Luminescence
- Clinical diagnostics
- Viral load
- HIV
- Hepatitis C
- Genetic research
See Also
External links
- Medical encyclopedia article on Branched DNA assay
- Wikipedia's article - Branched DNA assay
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