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Co amplification at lower denaturation temperature pcr

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Co amplification at lower denaturation temperature pcr - a modification of the standard polymerase chain reaction that increases the ability to preferentially amplify minority alleles and somatic mutations in the presence of an excess of wild type molecules. Sequence variant molecules are preferentially selected by the lower melting temperature of their mismatched heteroduplex after they've annealed to a wild type molecule that is present in excess. By carefully selecting the denaturing temperature below the tm for the wild type molecule, only the mismatch heteroduplexes will melt and act as efficient templates for strand synthesis.

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