Expression cassette

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Expression Cassette

An Expression Cassette (pronunciation: /ɪkˈsprɛʃən kəˈsɛt/) is a distinct component of a plasmid, or other vector system, that contains all the necessary elements for the transcription and translation of a cloned gene into a protein.

Etymology

The term "Expression Cassette" is derived from the field of molecular biology. "Expression" refers to the process by which information from a gene is used to create a functional product, typically a protein. "Cassette" refers to a segment of DNA that can be easily inserted or removed from a larger DNA molecule, much like a cassette tape can be inserted or removed from a tape player.

Components

An Expression Cassette typically includes the following components:

  • Promoter: A DNA sequence that initiates the process of transcription.
  • Gene of Interest: The gene that is to be expressed.
  • Terminator: A DNA sequence that signals the end of transcription.

Related Terms

  • Plasmid: A small, circular piece of DNA that is separate from the chromosomal DNA, often used as a vector in genetic engineering.
  • Vector (molecular biology): A DNA molecule used as a vehicle to transfer foreign genetic material into another cell.
  • Transcription (genetics): The process by which the information in a strand of DNA is copied into a new molecule of messenger RNA (mRNA).
  • Translation (genetics): The process by which mRNA is decoded and a protein is produced.

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