Disk diffusion test
Disk diffusion test
The Disk diffusion test, also known as the Kirby-Bauer test, is a method used in clinical microbiology to determine the susceptibility of microorganisms to antibiotics.
Pronunciation
- Disk: /dɪsk/
- Diffusion: /dɪˈfjuːʒən/
- Test: /tɛst/
Etymology
The term "disk diffusion test" is derived from the process itself. "Disk" refers to the antibiotic-impregnated disk used in the test, "diffusion" refers to the process by which the antibiotic spreads from the disk into the agar medium, and "test" refers to the method of determining antibiotic susceptibility.
Procedure
The disk diffusion test involves the following steps:
- A bacterial suspension is spread evenly over the surface of a Mueller-Hinton agar plate.
- Antibiotic-impregnated disks are placed on the agar surface.
- The plate is incubated, allowing the antibiotic to diffuse from the disk into the agar.
- The diameter of the zone of inhibition around each disk is measured and compared to standard tables to determine whether the organism is sensitive, intermediate, or resistant to each antibiotic.
Related Terms
- Antibiotic susceptibility testing: A set of procedures used to determine the sensitivity of bacteria to various antibiotics.
- Zone of inhibition: The area around an antibiotic disk where bacteria are unable to grow due to the presence of the antibiotic.
- Mueller-Hinton agar: A type of agar medium commonly used in antibiotic susceptibility testing.
- Clinical microbiology: The study of microorganisms, including bacteria, viruses, fungi, and parasites, that cause disease in humans.
See Also
External links
- Medical encyclopedia article on Disk diffusion test
- Wikipedia's article - Disk diffusion test
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