Knockin

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Knockin (pronounced: /ˈnɒkɪn/) is a term used in the field of genetics and molecular biology to refer to a method of creating specific target mutations in the DNA sequence of a living organism's genome.

Etymology

The term "knockin" is derived from the English words "knock" and "in". It metaphorically refers to the process of inserting a new gene into a specific location in an organism's genome.

Definition

A knockin is a genetic technique that involves the insertion of a functional gene at a specific locus in the genome. This is achieved through the use of recombinant DNA technology, where the desired gene is inserted into a vector, which is then introduced into the organism's cells. The cells' own repair mechanisms then incorporate the new gene into the genome at the desired location.

Related Terms

  • Gene Targeting: A method that uses homologous recombination to change an endogenous gene. It can be used to delete a gene, remove exons, add a gene, or introduce point mutations.
  • Knockout: A genetic technique that involves the creation of an organism that is genetically identical to the original, except for the absence of the gene being knocked out.
  • Transgenic Organisms: Organisms that have had a foreign gene deliberately inserted into their genome.
  • Homologous Recombination: A type of genetic recombination where nucleotide sequences are exchanged between two similar or identical molecules of DNA.

See Also

External links

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