Subcloning
Subcloning
Subcloning (pronunciation: /sʌbˈkləʊnɪŋ/) is a technique used in molecular biology to move a particular gene of interest from a parent vector to a destination vector.
Etymology
The term "subcloning" is derived from the prefix "sub-" meaning under or below, and "cloning", which refers to the process of producing similar populations of genetically identical individuals. In this context, it refers to the process of creating a subpopulation of clones from a parent vector.
Process
Subcloning involves several steps. First, the gene of interest is isolated from the parent vector using restriction enzymes. These enzymes cut the DNA at specific sequences, allowing the gene to be removed. The gene is then inserted into the destination vector, often using a process called ligation. This new vector can then be introduced into bacteria or other cells, where it can be replicated and studied.
Related Terms
- Cloning: The process of producing similar populations of genetically identical individuals.
- Vector: A DNA molecule used as a vehicle to artificially carry foreign genetic material into another cell.
- Restriction Enzymes: Proteins that scientists use to cut DNA at specific places.
- Ligation: The process of joining two pieces of DNA together.
- Bacteria: Single-celled microorganisms that can exist either as independent (free-living) organisms or as parasites (dependent on another organism for life).
Applications
Subcloning is used in various fields of research, including genetics, cell biology, and biochemistry. It allows scientists to study the function of specific genes, produce proteins, and create genetically modified organisms.
External links
- Medical encyclopedia article on Subcloning
- Wikipedia's article - Subcloning
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