Work loop

The work loop technique is used in muscle physiology to evaluate the mechanical work and power output of skeletal or cardiac muscle contractions via in vitro muscle testing of whole muscles, fiber bundles or single muscle fibers. This technique is primarily used for cyclical contractions such as the rhythmic flapping of bird wings or the beating of heart ventricular muscle.

To simulate the rhythmic shortening and lengthening of a muscle (e.g. while moving a limb), a servo motor oscillates the muscle at a given frequency and range of motion observed in natural behavior. Simultaneously, a burst of electrical pulses is applied to the muscle at the beginning of each shortening-lengthening cycle to stimulate the muscle to produce force. Since force and length return to their initial values at the end of each cycle, a plot of force vs. length yields a 'work loop'. Intuitively, the area enclosed by the loop represents the net mechanical work performed by the muscle during a single cycle.





History
Classical studies from the 1920s through the 1960s characterized the fundamental properties of muscle activation (via action potentials from motor neurons), force development, length change and shortening velocity. However, each of these parameters were measured while holding other ones constant, making their interactions unclear. For instance, force-velocity and force-length relationships were determined at constant velocities and loads. Yet during locomotion, neither muscle velocity nor muscle force are constant. In running, for example, muscles in each leg experience time-varying forces and time-varying shortening velocities as the leg decelerates and accelerates from heelstrike to toeoff. In such cases, classical force-length (constant velocity) or force-velocity (constant length) experiments might not be sufficient to fully explain muscle function.

In 1960, the work loop method was introduced to explore muscle contractions of both variable speed and variable force. These early work loop experiments characterized the mechanical behavior of asynchronous muscle (a type of insect flight muscle). However due to the specialized nature of asynchronous muscle, the work loop method was only applicable for insect muscle experiments. In 1985, Robert K. Josephson modernized the technique to evaluate properties of synchronous muscles powering katydid flight by stimulating the muscle at regular time intervals during each shortening-lengthening cycle. Josephson's innovation generalized the work loop technique for wide use among both invertebrate and vertebrate muscle types, profoundly advancing the fields of muscle physiology and comparative biomechanics.

Work loop experiments also allowed greater appreciation for the role of activation & relaxing kinetics in muscle power and work output. For instance, if a muscle turns on and off more slowly, the shortening and lengthening curves will be shallower and closer together, resulting in decreased work output. "Negative" work loops were also discovered, showing that muscle lengthening at higher force than the shortening curve can result in net energy absorption by the muscle, as in the case of deceleration or constant-speed downhill walking.

In 1992, the work loop approach was extended further by the novel use of bone strain measurements to obtain in vivo force. Combined either with estimates of muscle length changes or with direct methods (e.g. sonomicrometry), in vivo force technology enabled the first in vivo work loop measurements.

Positive, negative and net work
A work loop combines two separate plots: force vs. time and length vs. time. When force is plotted against length, a work loop plot is created: each point along the loop corresponds to a force and a length value at a unique point in time. As time progresses, the plotted points trace the shape of the work loop. The direction in which the work loop is traced through time is a critical feature of the work loop. As the muscle shortens while generating a tensile force (i.e. "pulling"), then, by convention in, the muscle is said to be performing positive work during that phase. As the muscle lengthens (while still generating a tensile force), the muscle is performing negative work (or, alternatively, that positive work is being performed  on the muscle). Thus a muscle generating force while shortening is said to output 'positive work' (i.e. generating work), whereas a muscle generating force while lengthening produces 'negative work' (i.e. absorbing work). Over an entire cycle, there is typically some positive, and some negative work; if the overall cycle is counter-clockwise vs. clockwise work loop represents overall work generation vs. work absorption, respectively. For example during a jump, the leg muscles generate work to increase the body's speed away from the ground, yielding counter-clockwise work loops. When landing, however, the same muscles absorb work to decrease the body's speed, yielding clockwise work loops. Furthermore, a muscle can produce positive work followed by negative work (or vice versa) within a shortening-lengthening cycle, causing a 'figure 8' work loop shape containing both clockwise and counter-clockwise segments.

Since work is defined as force multiplied by displacement, the area of the graph shows the mechanical work output of the muscle. In a typical work-generating instance, the muscle shows a rapid curvilinear rise in force as it shortens, followed by a slower decline during or shortly before the muscle begins the lengthening phase of the cycle. The area beneath the shortening curve (upper curve) gives the total work done by the shortening muscle, while the area beneath the lengthening curve (lower curve) represents the work absorbed by the muscle and turned into heat (done by either environmental forces or antagonistic muscles). Subtracting the latter from the former gives the net mechanical work output of the muscle cycle, and dividing that by the cycle duration gives net mechanical power output.

Inferring muscle function from work loop shape
Hypothetically, a square work loop (area = max force x max displacement) would represent the maximum work output of a muscle operating within a given force and length range. Conversely, a flat line (area = 0) would represent the minimum work output. For example, a muscle that generates force without changing length (isometric contraction) will show a vertical line 'work loop'. Reciprocally, a muscle that shortens without changing force (isotonic contraction) will show a horizontal line 'work loop'. Finally, a muscle can behave like a spring which extends linearly as a force is applied. This final case would yield a slanted straight line 'work loop' where the line slope is the spring stiffness.

Work loop experimental approach
Work loop experiments are most often performed on muscle tissue isolated either from invertebrates (e.g. insects and crustaceans ) or small vertebrates (e.g. fish, frogs, rodents ). The experimental technique described below applies both to in vitro and in situ approaches.

Experimental setup
Following humane procedures approved by IACUC, the muscle is isolated from the animal (or prepared in situ), attached to the muscle testing apparatus and bathed in Ringer's solution maintained at a constant temperature. While the isolated muscle is still living, the experimenter then applies two manipulations to test muscle function: 1) Electrical stimulation to mimic the action of a motor neuron and 2) strain (muscle length change) to mimic the rhythmic motion of a limb. To elicit muscle contraction, the muscle is stimulated by a series of electrical pulses delivered by an electrode to stimulate either the motor nerve or the muscle tissue itself. Simultaneously, a computer-controlled servo motor in the testing apparatus oscillates the muscle while measuring the force generated by the stimulated muscle. The following parameters are modulated by the experimenter to influence muscle force, work and power output:


 * Stimulation duration: The time period over which the muscle receives electrical stimulation
 * Stimulation pulse frequency: The number of stimulation pulses per stimulation duration
 * Stimulation phase: The time delay between the onset of stimulation and muscle length change
 * Strain amplitude: The difference between the maximum and minimum values of the length oscillation pattern
 * Strain frequency: The number of shortening-lengthening periods per time period



Calculating muscular work and power from experimental data
Calculation of either muscle work or power requires collection of muscle force and length (or velocity) data at a known sampling rate. Net work is typically calculated either from instantaneous power (muscle force x muscle velocity) or from the area enclosed by the work loop on a force vs. length plot. Both methods are mathematically equivalent and highly accurate, however the 'area inside the loop' method (despite its simplicity) can be tedious to carry out for large data sets.

Method 1: Instantaneous power method
Step 1) Obtain muscle velocity by numerical differentiation of muscle length data. Step 2) Obtain instantaneous muscle power by multiplying muscle force data by muscle velocity data for each time sample. Step 3) Obtain net work (a single number) by numerical integration of muscle power data. Step 4) Obtain net power (a single number) by dividing net work by the time duration of the cycle.

Method 2: Area inside the loop method
The area inside the work loop can be quantified either 1) digitally by importing a work loop image into ImageJ, tracing the work loop shape and quantifying its area. Or, 2) manually by printing a hard copy of the work loop graph, cutting the inner area and weighing it on an analytical balance. Net work is then divided by the time duration of the cycle to obtain net power.

Asymmetrical muscle length trajectories
Originally, workloops imposed a sinusoidal length change on the muscle, with equal time lengthening and shortening. However, in vivo muscle length change often has greater than half the cycle shortening, and less than half lengthening. Imposing these "asymmetrical" stretch-shorten cycles can result in higher work and power outputs, as shown in treefrog calling muscles.