Endonuclease

Endonuclease is a type of enzyme that functions by cleaving the phosphodiester bond within a polynucleotide chain. Some, such as deoxyribonuclease, act on DNA and others, such as ribonuclease, act on RNA.

Function

Endonucleases are essential for the survival of all organisms as they play a critical role in DNA replication, DNA repair, and genetic recombination. They are involved in processes such as mismatch repair to fix errors that occur during DNA replication and nucleotide excision repair to repair damage caused by external factors like ultraviolet light.

Types

There are two main types of endonucleases: restriction endonuclease and flap endonuclease. Restriction endonucleases recognize specific DNA sequences and cut at those sites, while flap endonucleases are involved in DNA replication and DNA repair.

Clinical Significance

Endonucleases have been used in molecular biology and genetic engineering for many years. They are used in techniques such as polymerase chain reaction (PCR), gene cloning, and gene sequencing. In medicine, they are being explored for use in gene therapy to treat genetic diseases.

See Also

• Exonuclease
• Nuclease
• DNA repair
• Genetic recombination

References

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Endonuclease

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  Restriction enzyme Eco RI

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  AP Endonuclease cartoon