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- ...orange in color. It is a lactam and an oxo heterocycle. It is a conjugate acid of an isatin(1-). Isatin is a derivative of indole with a carbonyl group at ...ethod, which involves oxidation of indigo dye with nitric acid and chromic acid. It can also be synthesized from [[aniline]] via the Sandmeyer reaction.1 KB (212 words) - 16:10, 4 February 2024