Elution
Elution
Elution (/ɪˈljuːʃən/) is a process in analytical chemistry used to separate components or analytes from a mixture. The term elution is derived from the Latin word "eluere", meaning to wash out or extract.
Etymology
The term "elution" originates from the Latin word "eluere", which means to wash out or extract. This term was first used in the context of chromatography, a technique in analytical chemistry, in the early 20th century.
Process
In the process of elution, a substance is washed through a column with a solvent, known as the eluent. The different components or analytes in the mixture have different affinities for the eluent, and thus move through the column at different rates. This allows for the separation of the components.
Types of Elution
There are two main types of elution: normal-phase elution and reversed-phase elution. In normal-phase elution, the eluent is a polar solvent, and the analytes with the highest affinity for the eluent elute first. In reversed-phase elution, the eluent is a nonpolar solvent, and the analytes with the lowest affinity for the eluent elute first.
Related Terms
- Analyte: The substance or chemical constituent that is of interest in an analytical procedure.
- Eluent: The solvent used in the process of elution.
- Chromatography: A technique used to separate mixtures.
- Normal-phase elution: A type of elution where the eluent is a polar solvent.
- Reversed-phase elution: A type of elution where the eluent is a nonpolar solvent.
External links
- Medical encyclopedia article on Elution
- Wikipedia's article - Elution
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