Brain heart infusion
Brain heart infusion (BHI) is a nutrient-rich medium used for the cultivation of a wide variety of microorganisms, including bacteria, yeasts, and molds. It is particularly useful in the cultivation of fastidious organisms that require enriched media for optimal growth.
Composition
Brain heart infusion is composed of several key ingredients that provide the necessary nutrients for microbial growth. These ingredients typically include:
- Calf brain infusion
- Beef heart infusion
- Peptone
- Dextrose
- Sodium chloride
- Disodium phosphate
- Distilled water
The exact composition can vary slightly depending on the manufacturer, but the essential components remain consistent to ensure the medium's effectiveness.
Preparation
To prepare BHI medium, the following steps are generally followed: 1. Measure the required amounts of each ingredient. 2. Dissolve the ingredients in distilled water. 3. Adjust the pH to the desired level, usually around 7.4. 4. Sterilize the medium by autoclaving at 121°C for 15 minutes. 5. Cool the medium to room temperature before use.
Applications
BHI is widely used in microbiology laboratories for various applications, including:
- Cultivation of Streptococcus species
- Growth of Listeria monocytogenes
- Isolation of fungi and yeasts
- Antibiotic susceptibility testing
- Research in microbial physiology and pathogenesis
Advantages
The advantages of using BHI medium include:
- Rich nutrient content that supports the growth of a wide range of microorganisms.
- Ability to cultivate fastidious organisms that may not grow well on other media.
- Versatility in various microbiological applications.
Limitations
Despite its advantages, BHI medium has some limitations:
- It may not be selective for specific microorganisms, leading to the growth of unwanted contaminants.
- The rich nutrient content can sometimes mask the presence of certain pathogens in mixed cultures.
See also
References
External links
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