MALDI imaging

MALDI Imaging

Matrix-Assisted Laser Desorption/Ionization Imaging (pronounced: /ˈmældi ɪˈmɪdʒɪŋ/), commonly known as MALDI Imaging, is a powerful analytical technique used in the field of mass spectrometry to visualize the spatial distribution of biomolecules in tissue sections.

Etymology

The term "MALDI" is an acronym for Matrix-Assisted Laser Desorption/Ionization, a soft ionization technique used in mass spectrometry. The term "Imaging" refers to the process of creating visual representations of the interior of a body for clinical analysis and medical intervention.

Definition

MALDI Imaging is a technique that combines the sensitivity and specificity of mass spectrometry with spatial fidelity. It allows for the direct analysis of biological samples, such as tissue sections, to produce images that represent the spatial distribution of molecules.

Procedure

The procedure of MALDI Imaging involves several steps. First, a thin layer of a matrix compound is applied to the tissue section. The matrix helps to protect the tissue and assists in the desorption and ionization of the molecules within the tissue. The tissue is then irradiated with a laser, causing the matrix and the tissue molecules to be desorbed and ionized. The ions are then analyzed by a mass spectrometer, and an image is created based on the distribution of specific ions.

Applications

MALDI Imaging has a wide range of applications in both research and clinical settings. It is used in proteomics, metabolomics, pharmacology, and pathology to study the distribution of proteins, peptides, lipids, drugs, and metabolites in tissues.

Related Terms

• Mass Spectrometry
• Matrix
• Proteomics
• Metabolomics
• Pharmacology
• Pathology

External links

• Medical encyclopedia article on MALDI imaging
• Wikipedia's article - MALDI imaging

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