RNA-Seq
RNA-Seq
RNA-Seq (RNA sequencing) is a sequencing technique used to reveal the presence and quantity of RNA in a biological sample at a given moment.
Pronunciation
RNA-Seq: /ɑːrɛnˈeɪsɛk/
Etymology
The term "RNA-Seq" is a portmanteau of "RNA" and "sequencing". "RNA" stands for ribonucleic acid, a nucleic acid present in all living cells. The "Seq" is short for "sequencing", referring to the process of determining the precise order of nucleotides within a DNA molecule.
Definition
RNA-Seq is a high-throughput sequencing technique that uses next-generation sequencing (NGS) to reveal the presence and quantity of RNA in a biological sample at a given moment. This technique is used to analyze the continuously changing cellular transcriptome, which is the set of all RNA molecules in one cell or a population of cells.
Related Terms
- Transcriptome: The set of all RNA molecules, including mRNA, rRNA, tRNA, and other non-coding RNA, produced in one or a population of cells.
- Next-Generation Sequencing (NGS): A high-throughput DNA sequencing technology that has revolutionized genomic research.
- Ribonucleic Acid (RNA): A polymeric molecule essential in various biological roles in coding, decoding, regulation and expression of genes.
- DNA Sequencing: The process of determining the nucleic acid sequence – the order of nucleotides in DNA.
See Also
External links
- Medical encyclopedia article on RNA-Seq
- Wikipedia's article - RNA-Seq
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