Dark-field microscope
Dark-field Microscope
A Dark-field microscope (pronunciation: /dɑːrk fiːld ˈmaɪkrəskoʊp/) is a type of microscope that utilizes a dark-field illumination method to enhance the contrast in unstained, transparent samples.
Etymology
The term "Dark-field microscope" is derived from the fact that the specimen is made to appear light against a dark background, which is the opposite of what is seen in normal light microscopy.
Description
In a Dark-field microscope, light is directed at an angle to the sample, which prevents direct, unscattered light from entering the objective lens. This results in the specimen appearing bright against a dark background. This method is particularly useful for observing living organisms, such as bacteria, that would be difficult to see using other methods.
Related Terms
- Bright-field microscopy: A common method in light microscopy that illuminates the sample evenly with a bright field of light.
- Phase contrast microscopy: A type of optical microscopy that uses phase shifts in light passed through a transparent specimen to enhance contrast.
- Fluorescence microscopy: A type of light microscope that uses fluorescence and phosphorescence instead of, or in addition to, reflection and absorption to study properties of organic or inorganic substances.
See Also
External links
- Medical encyclopedia article on Dark-field microscope
- Wikipedia's article - Dark-field microscope
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