Jaffe reaction
Jaffe Reaction
Jaffe reaction (pronunciation: /ˈjæf.i rɪˈæk.ʃən/) is a biochemical method used to measure the concentration of creatinine in the blood serum, urine, and other body fluids.
Etymology
The Jaffe reaction is named after the German chemist Max Jaffé (1841–1911), who first described the method in 1886.
Method
The Jaffe reaction involves the reaction of creatinine with picric acid in an alkaline solution to form a red-orange complex. The intensity of the color, which can be measured using a spectrophotometer, is directly proportional to the concentration of creatinine in the sample.
Related Terms
- Creatinine: A waste product produced by the normal breakdown of muscle tissue.
- Picric acid: A chemical reagent used in the Jaffe reaction.
- Spectrophotometer: An instrument used to measure the intensity of light in a part of the spectrum, especially as transmitted or absorbed by particular substances.
Limitations
The Jaffe reaction is not specific for creatinine and can be influenced by other substances present in the sample, such as glucose, ketones, and certain drugs. This can lead to overestimation of the creatinine concentration.
See Also
External links
- Medical encyclopedia article on Jaffe reaction
- Wikipedia's article - Jaffe reaction
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