Solid phase extraction
Solid Phase Extraction
Solid Phase Extraction (SPE) /ˈsɒlɪd feɪz ɪkˈstrækʃən/ is a sample preparation technique used in analytical chemistry for the separation and concentration of analytes from a sample solution. The technique is based on the principle of liquid chromatography.
Etymology
The term "Solid Phase Extraction" is derived from the process itself. "Solid Phase" refers to the solid medium (the sorbent) that is used to extract the analytes. "Extraction" refers to the process of removing the analytes from the sample.
Process
The process of Solid Phase Extraction involves four main steps:
- Conditioning: The sorbent is activated by passing a solvent through it.
- Loading: The sample is passed through the sorbent, and the analytes are retained.
- Washing: Unwanted components are removed from the sorbent.
- Elution: The analytes are removed from the sorbent using a suitable solvent.
Applications
Solid Phase Extraction is widely used in various fields such as environmental analysis, forensic science, biochemistry, and pharmaceutical industry. It is particularly useful for the extraction of trace amounts of analytes from large volumes of sample.
Related Terms
External links
- Medical encyclopedia article on Solid phase extraction
- Wikipedia's article - Solid phase extraction
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