Wayson stain
Wayson Stain
Wayson Stain (pronunciation: /ˈweɪsən steɪn/) is a type of histological stain used primarily in the field of microbiology and pathology.
Etymology
The term "Wayson Stain" is named after its developer, Dr. George Wayson, an American bacteriologist who introduced this staining method in the early 20th century.
Description
Wayson Stain is a polychrome methylene blue solution used for staining certain types of bacteria, particularly Yersinia pestis, the bacterium responsible for plague. It is also used to stain other organisms such as Francisella tularensis, the causative agent of tularemia. The stain allows for the visualization of bipolar staining, a characteristic feature of these bacteria.
Procedure
The procedure for Wayson Stain involves applying the stain to a smear of the sample on a microscope slide. The slide is then heated gently and rinsed with water. The stained slide is then examined under a microscope. The bacteria, if present, will appear as dark blue or purple rods against a light blue background.
Related Terms
- Histological Stain: A type of stain used in microscopy to enhance contrast in the microscopic image.
- Yersinia pestis: A gram-negative bacterium that can infect humans via the oriental rat flea. It is the causative agent of plague.
- Francisella tularensis: A pathogenic species of Gram-negative bacteria, which is the causative agent of tularemia.
- Bipolar Staining: A staining technique that allows certain types of bacteria to be visualized as having a concentration of stain at each end, or pole, of the cell.
External links
- Medical encyclopedia article on Wayson stain
- Wikipedia's article - Wayson stain
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